Serodiagnosis of syphilis in the recombinant era: reversal of fortune.
نویسندگان
چکیده
Karen W. Hoover1 and Justin D. Radolf2 1Division of Sexually Transmitted Disease Prevention, National Center for HIV/AIDS, Viral Hepatitis, Sexually Transmitted Disease, and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia; and 2Departments of Medicine, Pediatrics, Immunology, and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington
منابع مشابه
Serodiagnosis of syphilis: antibodies to recombinant Tp0453, Tp92, and Gpd proteins are sensitive and specific indicators of infection by Treponema pallidum.
Syphilis serodiagnosis relies on a combination of nonspecific screening tests (antilipoidal antibodies) and Treponema pallidum-specific tests (anti-T. pallidum antibodies). We studied a group of six recombinant T. pallidum antigens for their sensitivities and specificities with sera from individuals with syphilis (n = 43), relapsing fever (n = 8), Lyme disease (n = 8), and leptospirosis (n = 9)...
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Visceral leishmaniasis (VL) is a zoonotic disease caused by leishmania species. Dogs are considered to be the main reservoir of VL. A number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. However, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. A variety of serological tests ...
متن کاملImportance of L. Infantum H2B Recombinant Antigen for Serodiagnosis of Visceral Leishmaniasis
Background: Visceral leishmaniasis (VL) can lead to death in more than 95% of cases if left untreated. Accurate and early diagnosis has an important role in reducing mortality rate of this disease. Objective: To express recombinant H2B antigen from an Iranian isolate of Leishmania Infantum and evaluate its efficacy in the diagnosis of VL. Metho...
متن کاملEvaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis.
Syphilis is a chronic infection caused by Treponema pallidum subsp. pallidum, and diagnosis with sensitive and specific methods is a challenging process that is important for its prevention and treatment. In the present study, we established a recombinant protein TpF1-based indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and a Western blot assay for human and rabbit se...
متن کاملEnzyme-Linked Immunosorbent Assay Using the Recombinant DNA-Derived Treponema pallidum Protein TmpA for Serodiagnosis of Syphilis and the Potential Use of TmpA for Assessing the Effect of Antibiotic Therapy
The recombinant DNA-derived Treponema pallidum membrane protein TmpA, purified from Escherichia coli K-12, was used in an enzyme-linked immunosorbent assay (ELISA) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. The sensitivity of the TmpA ELISA was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syp...
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ورودعنوان ژورنال:
- The Journal of infectious diseases
دوره 204 9 شماره
صفحات -
تاریخ انتشار 2011